Purification and characterization of smooth muscle cell caveolae.
نویسندگان
چکیده
منابع مشابه
Purification and characterization of smooth muscle cell caveolae
Plasmalemmal caveolae are a membrane specialization that mediates transcytosis across endothelial cells and the uptake of small molecules and ions by both epithelial and connective tissue cells. Recent findings suggest that caveolae may, in addition, be involved in signal transduction. To better understand the molecular composition of this membrane specialization, we have developed a biochemica...
متن کاملAssociation of smooth muscle cell tissue factor with caveolae.
There is still no satisfactory explanation for the low catalytic activity of tissue factor (TF)/factor VII(a) complexes towards coagulation factor X, as found on the apical surface side of cell layers. It has been hypothesized that TF exists in a latent form. Layers of cultured human smooth muscle cells, constitutively expressing TF, were immunogold-labeled for TF in situ and processed for elec...
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Smooth muscle myosin light chain kinase was purified from turkey gizzards. The enzyme was extracted from washed myofibrils and the final step of purification was affinity chromatography using calmodulin coupled to Sepharose 4B. The purified enzyme was characterized with respect to its physical, chemical, and kinetic properties. It has a molecular weight of 130,000 by sodium dodecyl sulfate poly...
متن کاملCaveolae and propofol effects on airway smooth muscle.
BACKGROUND The i.v. anaesthetic propofol produces bronchodilatation. Airway relaxation involves reduced intracellular Ca(2+) ([Ca(2+)](i)) in airway smooth muscle (ASM) and lipid rafts (caveolae), and constitutional caveolin proteins regulate [Ca(2+)](i). We postulated that propofol-induced bronchodilatation involves caveolar disruption. METHODS Caveolar fractions of human ASM cells were test...
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In the last 15 years several electron-microscope studies of visceral and vascular smooth muscle of vertebrates have been published (see reviews by Dewey & Barr, 1968, and Burnstock, 1970). The presence of bottle-shaped invaginations of the sarcolemmal membrane (micropinocvtotic vesicles, caveolae intracellulares) (Caesar, Edwards & Ruska, 1957) is well known. However, it is generally accepted t...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1994
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.126.1.127